An improved photochemical method for the rapid spectrophotometric detection of superoxide dismutase

A modified spectrophotometric assay of superoxide dismutase.

A simple and rapid method for the assay of superoxide dismutase in biological samples is described. Present method takes advantage of the inhibition of NADH-dependent-nitroblue tetrazolium reduction by the dismutase. Inhibition of the chromogen formation by superoxide dismutase was linear with increase in enzyme concentrations. The chromogen extract in butanol was stable even up to 48 hr. Super...

Modifying superoxide dismutase for improved biopharmaceutical properties

The superoxide dismutase enzyme (SOD) contributes to the physiological equilibrium between pro-oxidants and antioxidants, by destroying the superoxide anion (O2). However, its therapeutic use is limited by its fast clearance from the bloodstream and inactivation by its own reaction product, i.e. hydrogen peroxide. Here is a summary of the main strategies developed to circumvent these limitation...

An Improved GC Method for Rapid Analysis of Valproic Acid in Human Plasma Without Derivatization

        A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®<span styl...

Superoxide dismutase Superoxide dismutase

An improved method for geological boundary detection of potential field anomalies

Potential field methods such as gravity and magnetic methods are among the most applied geophysical methods in mineral exploration. A high-resolution technique is developed to image geologic boundaries such as contacts and faults. Potential field derivatives are the basis of many interpretation techniques. In boundary detection, the analytic signal quantity is d...